【摘要】 目的 比较不同提取方法制备的养血软坚方提取物中指标成分的含量及其抗炎镇痛作用,确定养血软坚方的提取方法。方法分别采用常规水煎提取、乙醇回流提取和乙醇渗漉提取法制备养血软坚方的提取物,以芍药苷和龙胆苦苷为化学指标成分,采用HPLC测定其含量,并以热板法和小鼠耳廓二甲苯致炎模型评价不同提取方法制备的提取物的镇痛和抗炎作用。结果 3种方法制备的提取物中龙胆苦苷含量基本相等,而芍药苷在醇渗漉提取物中含量明显高于醇回流提取物,水煎提取物中含量最低。水煎提取物镇痛作用最强,醇回流提取物次之,醇渗漉提取物最弱;三者的抗炎作用相似。 结论 养血软坚方以水煎提取方法为宜,复方中药提取工艺的选择需结合复方自身的特点。更多还原

【Abstract】 Objective To establish a proper extraction method for Yangxue Ruanjian Prescription (YRP) by comparing the chemical component contents and analgesic and anti - inflammatory effect of extracts extracted from YRP by different methods. Methods Water - decocting, alcohol - refluxing and alcohol - percolating methods were adopted and component indexes of paeoniflorin and gentiopicrin were determined by HPLC. The anti - inflammatory effect of the extracts were observed on mice with xylol - induced auricular swelling and analgesic effect by hot - plate method. Results The content of gentiopicrin is similar in the extracts extracted by three methods and the content of paeoniflorin is higher in alcohol - percolating extract than in alcohol - refluxing extract, lowest in water - extraction. The analgesic effect of water - decocting extract is better than that of alcohol - refluxing extract, and that of alcohol - percolating extract is weakest; the anti - inflammatory effect of the three extracts is almost the same. Conclusion Water - decocting extraction is suitable to YRP, and this is determined by the characteristics of Chinese compound prescription.更多还原