【摘要】 目的 :采用高效液相色谱法测定梦宁片中栀子苷含量。方法 :采用EclipseXDB C18色谱柱 (4 .6mm× 2 5 0mm ,5μm) ,以0 .2 %三乙胺水溶液 (含 0 .1%磷酸 ) 乙腈 (85∶15 )为流动相 ,检测波长为 2 4 0nm ,流速 1.0mL·min-1。结果 :栀子苷进样量在 0 .0 988μg～0 .4 94 0 μg范围内与峰面积积分值具有良好的线性关系 ,相关系数r =0 .9995 (n =5 ) ,回收率为97.91% (n =5 )。结论 :本方法操作简便 ,精密度好 ,结果准确可靠。更多还原

【Abstract】 Objective: To determine geniposide in a Chinese patent medicine Mengning tablets by HPLC.Method: The eclipse XDB-C 18 column ( 4.6 mm×250 mm,5 μm) with a mobile phase consisting of 0.2% triethylamine (containing 0.1% phosphate)-acetonitrile (85∶15) at a flow rate of 1.0 ml·min -1 and detected at 240 nm. Result: The calibration curve was linear within a range of 0.0988～ 0.4940 μg for geniposide (r= 0.999 5,n=5). The average recovery was 97.91%(n=5).Conclusion: The method is rapid, accurate and reliable.更多还原