【摘要】 目的 :建立法莫替丁咀嚼片含量测定的RP HPLC。方法 :采用ShimpackODS柱 (1 50mm× 6mm ,5μm) ,流动相为 :0 .1 %三乙胺的 0 .1mol·L- 1 乙酸钠溶液 (pH6 .0 0 )∶乙腈 (92∶8) ;流速 1 .0ml·min- 1 ,检测波长 2 66nm。结果 :在选定的色谱条件下 ,法莫替丁与有关物质完全分离 ;制剂中辅料对主药无干扰 ,法莫替丁在 5～ 2 0 0 μg·ml- 1 浓度范围内 ,峰面积与浓度线性关系良好 ,相关系数r =0 .9999,平均回收率为 99.4% ,RSD =0 .9%。结论 :本方法可用于法莫替丁咀嚼片的含量测定和有关物质检查。更多还原

【Abstract】 Objective: To establish a RP HPLC method to determine the content of famotidine chewable tablet.Method: Shimpack ODS column(150 mm×6 mm, 5 μm)was used, mobile phase was 0.1% v/v of triethylamine 0.1 mol·L -1 of sodium acetate (pH 6.00 ) (92∶8), flow rate was 1.0 ml·min -1 , detecting wavelength was at 266 nm. Result: Famotidine was completely separated from the relative substances in the selected conditions of chromatogram, the main drug had not been interfered by rehicles in preparation. The standard curve in linear within a rage of 5 200 μg·ml -1 with a correlation coefficient of 0.9999 , the average recovery was 99.4% ( RSD = 0.9% ).Conclusion: The determination method is simple and rapid by RH HPLC, famotidine in this product can be controlled.更多还原