【摘要】 目的从分子水平观察肿瘤血管生成因子VEGF、VEGF受体KDR、基质金属蛋白酶-1(MMP-1)及其转录调节因子Ets-1(E26transformation-specific-1,Ets-1)在子宫颈癌中的表达并揭示其因子间相互关系,为判断患者预后和解释促血管生成因子间内在规律提供根据。方法利用原位杂交和免疫组织化学染色法检测87例子宫颈癌组织中VEGF、KDR、MMP-1以及Ets-1等因子mRNA和蛋白表达规律;患者生存曲线比较在阴性组和阳性组间进行;因子间分析利用Pearson相关分析法。结果VEGFmRNA和蛋白主要分布在癌细胞胞质中,阳性率分别为78.6%(68/87)和70.4%(61/87);KDRmRNA和蛋白主要在内皮细胞上表达,其表达率与VEGF表达率间存在密切的相关关系,r=0.892;MMP-1在肿瘤细胞和间质血管内皮细胞中表达,尤其在血管新生处表达明显;Ets-1主要分布在内皮细胞和肿瘤细胞中,其表达率与KDR和MMP-1表达率间的相关系数(r)分别为0.900和0.873;上述四因子表达与肿瘤分化度、淋巴结转移及5年存活率均有密切的相关性。结论VEGF、KDR、MMP-1以及Ets-1是参与子宫颈癌血管生成的重要因子,且彼此间存在密切的相关性,检测其表达规律可为判断子宫颈癌生物学行为和解释促血管生成因子间内在联系提供理论根据。更多还原

【Abstract】 To study the expression of VEGF,KDR,MMP -1,and its transcription factor Ets-1on the interstitial neovasculogenesis in human cervical carcinoma on molecular level,which may provide further theoretic bases on judgement of prognosis and explain interregularity between neovasculagenetic factors.Methods VEGF,KDR,MMP -1,and Ets-1were detected in87cervical carcinomas by in situ hybridization and immunohistochemistry.The survival curves for patients with detected tumors were compared with the curves for those with out tumors.Multivariate analyses were performed with Pearson analysis.Results VEGF mRNA and its protein were mainly expressed in cytoplasms of tumor cells,positive rate was78.6%(68/87)and70.4%(61/87)respectively.KDR mRNA and its protein were mainly expressed in vascular endothelial cells,as correlation coefficient between KDR and VEGF was0.892.Over expression of MMP -1was seen in both endothelial cells and tumor cells,especially in hyperplasia of endothelial cells.Ets-1was mainly expressed in both endothelial cells and tumor cells,correlation coefficient between Ets-1and KDR,MMP -1,was0.900and0.873,respectively.Four factors of above were highly related with tumor differentiation,lymph nodes metastasis as well as5years survival rate.Conclusions VEGF,KDR,MMP -1,and Ets-1are important factors on neovasc ulogenesis in cervical carcinoma,which may be considered to be reference indicator for determining biology behavior of cervical carcinoma,and may provide further theoretic bases on antineova sculagenetic therapy.更多还原