【摘要】 目的观察苯甲酸雌二醇对人正常乳腺的影响。方法将9只裸鼠随机分为A、B、C3组,每组3只。取3例人正常乳腺组织标本,并将每例标本分为3份,分别植入各组中的1只裸鼠。植入2周后,从A组取出部分植入组织作对照组,之后A、B、C组裸鼠开始分别肌肉注射苯甲酸雌二醇3、6、9μg,每天1次,共28d;后处死裸鼠,取出植入组织。采用免疫组化方法检测各组植入组织的增殖细胞核抗原(proliferatingcellnuclearantigen,PCNA)、雌激素受体(estrogenreceptor,ER)、孕激素受体(progesteronereceptor,PR)的蛋白表达。结果与对照组相比,B、C组乳腺上皮细胞PCNA表达明显升高(P<0.05),A组乳腺PCNA无明显改变。随苯甲酸雌二醇剂量升高,ER表达逐渐下降(P<0.05),PR表达则逐渐上升(P<0.05)。结论一定剂量的苯甲酸雌二醇可促进正常乳腺的增生。更多还原

【Abstract】 To investigate the proliferating effect of estradiol benzoate on normal human breast tissue.Methods Three specimens of normal human breast tissue were implanted into nine9～10-week-old intact female athymic nude mice which were randomly divided into group A,B and C.Each specimen was divided into three parts,and each part was implanted into mice of each group of three(A,B and C)respectively.Two weeks later,part of xenografts was taken out from group A as control group,and then group A,B and C were injected musclularly with3,6,9μg estradiol benzoate once daily,respectively.After therapy for28days,the grafts were taken out and expression of proliferating cell nuclear antigen(PCNA),estrogen receptor(ER)and proliferating receptor(PR)in grafts were examined using immunohistochemical method.Results Compared with control group,PCNA was increased in group B and C(P<0.05),but in group A had no significant change.ER was gradually down regulated by3,6and9μg estradiol benzoate(P<0.05);however,PR was increasingly up regulated(P<0.05).Conclusions Certain dosage of estradiol benzoate has proliferating effect on normal human breast tissue.更多还原