【摘要】 目的:探讨沙棘籽渣黄酮类化合物(FSH)对人肝癌细胞BEL-7402的抑制和诱导凋亡作用。方法:采用SRB观察FSH对人肝癌细胞BEL-7402的生长抑制作用,采用wright染色、透射电镜和流式细胞术检测FSH对BEL-7402细胞的促凋亡作用。结果:200～1200μg/ml FSH能够剂量依赖性地抑制 BEL-7402细胞的增殖,wright染色观察到凋亡小体,透射电镜下发生染色质边集,流式细胞术检测出现亚二倍体峰。结论:FSH能够抑制BEL-7402的生长并能诱导细胞凋亡。更多还原

【Abstract】 Objective:To investigate the inhibitory and apoptosis-inducing effects of flavonoids from oil-removed seeds of Hippophae rhamnoides ( FSH) on liver cancer cell line BEL-7402. Methods: SRB , Wright staining, electron microscope and flow cytometry are used to study the effects of STT on BEL-7402 cell line. Results: FSH has potent inhibitive effect on BEL-7402 cell line in a concentration-dependent manner. BEL-7402 cells exibit typical morphological alteration of apoptosis when sub-Gl peak can be seen. Conclusion: FSH exerts its inhibitive effect on BEL-7402 cells by inducing apoptosis.更多还原