【摘要】 目的 :研究皮下植入依托泊苷的血药浓度测定方法。方法 :血浆样品以氯仿萃取 ,常温下负压挥发至干后 ,然后以流动相溶解 ,HPLC法测定。流动相为甲醇 水 ( 5 2∶48)的混合溶液 ,检测波长为 2 2 8nm ,色谱柱为LichrospherC18柱 ( 2 5 0mm× 4.6mm ,5 μm)。用外标法定量。此外进行了血浆样品的保存稳定性试验。 结果 :检出限为 0 .0 2 μg·mL-1,绝对回收率为 80 .4% ,相对回收率为 94.7% ,日内和日间精密度RSD均约 8% ,依托泊苷与血浆成分 (内源性干扰物 )达到良好分离。血浆样品萃取后残渣于 - 5～ 0℃条件可保存 2 40h。结论 :本方法灵敏度较高 ,准确性和实用性好。更多还原

【Abstract】 Objective:To establish the method for determination of plasma etoposide concentration after implanted subcutaneously.Methods:The plasma sample was extracted with chloroform,and vaporized at normal temperature in negative air pressure,and was determined by HPLC.The mobile phase was the mixture of methanol-water(52∶48)and the detection wavelength was set at 228nm.The sample was separated on Lichrospher C 18 column(250 mm×4.6mm,5μm)and determined with external standard method. Results :The detection limit was 0.02μg· mL -1 .The absolute recovery rate was 80.4%; the relative recovery rate was 94.7%. Intra-day and inter-day RSD were both about 8%.Under the condition etoposide was well separated from the fractions of plasma.The stability test of plasma sample showed that the solid crystallize was stable under the preservative temperature of -5～0℃ for 240 hours. Conclusion :This method is exact,sensitive,and practical for determination of plasma concentration of etoposide after implantde subcutaneously in clinic.更多还原