【摘要】 目的 :观察家兔晶状体后囊膜在人工晶状体植入后及柔红霉素作用下的组织形态学变化。方法 :家兔 2 0只被随机分为两组 :实验组 13只 ,对照组 7只 ,均行白内障囊外摘除人工晶状体植入术。实验组术前于晶状体前囊下注入 8mg/L柔红霉素 0 .2ml,对照组注射同量乳酸林格液 ,作用 5min。分别于术后 7d、14d、2 8d时处死两组家兔 ,每个收稿日期 :2 0 0 2 -10 -2 2 ;修回日期 :2 0 0 3 -0 1-15基金项目 :杭州市卫生局科研基金项目。作者简介 :任晓冰 (1969-) ,女 ,主治医生 ,研究方向 :肿瘤病理。通信作者 :任晓冰 (E -mail:xiaoren @mail.hz.zj.cn)时段实验组均取 4只 ,对照组均取 2只 ;18只眼球行后囊膜形态学观察并比较细胞密度 ;两组各有 1只于术后 2 8d处死 ,行后囊膜透射电镜观察。结果 :7d时两组后囊晶状体上皮细胞增殖 ,14d时实验组后囊膜细胞密度 (7.6± 2 .8)与对照组 (12 .8± 4 .8)相比降低 (P =0 .0 39) ,2 8d时实验组后囊膜细胞退变 ,密度进一步降低 (实验组 3.5± 0 .9,对照组 9.3± 1.3,P =0 .0 0 1)。电镜下对照组细胞结构完整 ;实验组细胞退变 ,核质溶解。结论 :家兔白内障术后晶状体后囊膜细胞发生增殖 ,柔红霉素短期内能够抑制家兔晶状体后囊膜细胞增殖。更多还原

【Abstract】 Objective:To evaluate the histopathological features of posterior capsule cells in the rabbit lens after intraocular lens surgery and under the influence of daunomycin.Methods:Twenty rabbits were randomly assigned to two groups:control group and experimental group namely,for ECCE(extra capsular cataract extraction)+IOL(intraocular lens) surgery. Before the lens capsule was truncated,0.2 ml of daunomycin(8 mg/L) was injected into the anterior capsule in the experimental group;while the same volume of lactic acid ringer’s solution was used in the control group. After waiting 5 minutes for the solution to permeate to the ambitus,the routine surgery was completed. 18 rabbits were sacrificed 7,14 and 28 d after surgery,and the posterior capsule cells of the lens were observed under light microscopy. 2 rabbits were sacrificed 28 d after surgery and the posterior capsules were observed under a transmission electron microscope(TEM).Results:Greater fibrosis and vacuolated cell proliferation of the lens epithelial cells could be seen 7,14 and 28 d after surgery in the control group. The situation was similar in the experimental group 7 d postoperatively. However,14 d postoperatively rare faction fibrosis and vacuolated cell proliferation could be seen in the posterior capsule cells of the experimental group and the nuclei degenerated with obviously lowered cell density(7.6±2.8,12.8±4.8,P=0.039). 28 d postoperatively the nuclei progressively degenerated with low cellular density(3.5±0.9,9.3±1.3,P=0.001). Under TEM,the cells in the control group were tightly arrayed with clearly defined cellular boundary and structure;while cellular deformity and undefined intracellular structure and nuclear cytoplasmic lysis could be seen in the experimental group.Conclusion:After intraocular lens surgery,the posterior capsule cells of the rabbit lens can proliferate. Daunomycin can inhibit this proliferation.更多还原