【摘要】 目的　研究当归多糖 (ASP)对鼠实验性结肠炎中氧自由基 (OFR)的影响。方法　通过建立大鼠TNBS性结肠炎模型 ,检测超氧化物歧化酶 (SOD)、谷胱甘肽过氧化酶 (GSH -Px)和丙二醛 (MDA)的含量变化 ,以反映大鼠结肠组织中氧自由基的改变及不同剂量的当归多糖对其影响 ,并以髓过氧化物酶 (MPO)及病理组织学损伤评分 (HS)分别评价结肠组织炎症程度及粘膜损伤程度。结果　模型组SOD、GSH -Px显著降低 ,MDA、MPO和HS明显升高 ,SOD、GSH -Px与MPO成负相关 ,MDA、HS与MPO成正相关。不同剂量的ASP均可不同程度地改善模型组各项检测指标。结论　ASP具有一定清除OFR的能力 ,减轻结肠炎症反应及组织损伤。更多还原

【Abstract】 Objective:To investigate the effects of Angelica Sinensis polysaccharide(ASP) on oxygen free radicals of experimental colitis in rats.Methods:The classic colitis model of rats was induced by intracolon enema with 2,4,6-trinitrobenzene sulfonic acid(TNBS) and ethanol. The experimental animals were randomly categorized into 5 groups: normal group, model group, and 3 ASP groups, treated intracolicaly with saline, saline and ASP(250,500,1?000?mg/kg) respectively and daily for 21 days. The myeloperoxidase(MPO) activity and the levels of SOD MDA and GSH-Px in the colon tissue were measured. Meantime, histopathological score (HS) of the colon tissue was evaluated.Results:The activities of SOD and GSH-Px were significantly decreased while the MDA content, MPO activity and HS were remarkably elevated in model group. ASP not only increased the activities of SOD and GSH-Px but also reduced the MDA content and MPO activity in a dose-dependent manner in model group. ASP also ameliorated the HS.Conclusions:ASP have protective effect on colon injury of experimental colitis rats, which may be related with its anti-oxidation property.更多还原