【摘要】 目的:通过检测膀胱移行细胞癌(BTCC)中E-Selectin、E-Selectin mRNA的表达结果,探讨其表达与肿瘤分级、分期的关系,并初步探讨其临床意义。方法:以免疫组化SABC法及原位杂交法分别检测32例膀胱移行细胞癌中E-Selectin、E-Selectin mRNA的表达,分析其与肿瘤分期、分级的关系。结果:32例膀胱移行细胞癌组织中23例E-Selectin mRNA的表达阳性,占71.8％,9例表达阴性,占28.2％;20例E-Selectin表达阳性,占62.5％,12例表达阴性,占37.5％;其表达与肿瘤的分级无关(P>0.05),但与肿瘤的临床分期明显相关(P<0.05);11例复发的BTCC患者中,11例E-Selectin mRNA表达阳性,10例E-Selectin表达阳性,其表达与肿瘤的复发显著相关。结论:在膀胱移行细胞癌中,E-Selectin及E-Selectin mRNA的表达明显上调,可能促进了膀胱移行细胞癌的血源性转移,且其表达与肿瘤的分期及复发有明显关系,可能成为判断膀胱移行细胞癌预后的一项重要指标。更多还原

【Abstract】 Objective:To make a preliminary study of the expression of the E - Selectin mRNA and E - Selectin in bladder transitional cell carcinoma (BTCC), and then their possible clinic significance. Methods:32 fresh surgical specimens of BTCC were examined by immunohistochemistry for E - Selectin protein expression and in situ hybridization for E - Selectin mRNA expression. We discussed the correlation between the expression of E - Selectin mRNA, E- Selectin and tumor clinical stage and tumor pathological grade. Results:23/32 tumor specimens had positively expressed E- Selectin mRNA,20/32 tumor specimens showed positive E- Selectin expression. Abnormal expression of E- Selectin mRNA and E - Selectin had a significantly association with tumor clinical stage but not with increased tumor grade. 11 recurrent tumor specimens showed positive expression of E- Selectin mRNA, of which 10 tumors showed positive expression of E - Selectin. The abnormal expression had a significant association with tumor recurrence. Conclusions: The over - expression of E - Selectin and E - Selectin mRNA is frequent in BTCC. There is a significant correlation between the over - expression of E - Selectin, E - Selectin mRNA and metastasis , recurrence in BTCC. The over - expression of E - Selectin and E - Selectin mRNA may be potential prognostic markers in BTCC.更多还原