【摘要】 目的　研究在高频率刺激及压迫追加的动态负荷下双节段受压马尾神经电生理的变化 ,探讨神经源性间歇跛行的发病机制。　方法　 SD大鼠 30只 ,体重 (32 9± 12 ) g,平均分成 5组。对照组行单纯 L5椎板切除 ;其他 4个实验组将特制硅胶片插入 L4 及 L6 椎管 ,造成横截面积缩小 30 %的双节段狭窄。施加静态压迫 2小时后 ,实验 1组不施加动态负荷 ;实验 2组只施加高频率刺激 6分钟 ;实验 3组同时施加高频率刺激和压迫追加 6分钟 ;实验 4组只行压迫追加 6分钟。动态负荷 6分钟后各组均恢复到原始静态受压状态继续检测 30分钟。　结果　静态压迫 2小时后 ,各实验组的运动及感觉神经传导速度均较压迫前明显下降 ,各组间差异无统计学意义 (P<0 .0 5 )。对照组无明显变化。实验 1组在实验最后 30分钟 ,运动及感觉神经传导速度变化无统计学意义 ,而其他 3个实验组在动态负荷后 ,运动及感觉神经传导速度均出现了不同程度下降 ,以实验 3组的变化最明显。　结论　静态受压的马尾神经在动态负荷下神经传导速度明显下降 ,同时施加两种动态负荷比施加单一负荷变化更明显。更多还原

【Abstract】 Objective To study the changes of neural electrophysiology properties of cauda equina under double level compression and dynamic burdens, and to clarify the mechanisms of intermittent neurogenic claudication. Methods Thirty SD rats were divided into 5 groups( 6 in each group). The laminectomy of L 5 was performed in control group. In the experimental groups, the silicon sheets were inserted into the spinal canal of L 4 and L 6 to cause double level compression of cauda equina by 30%. Two hours after onset of compression, no dynamic burden was introduced in experimental group 1. Only high frequency stimulation(HFS) was introduced for 6 minutes in experimental group 2. Both HFS and additional increased compression were introduced for 6 minutes in experimental group 3. While only additional increased compression was introduced for 6 minutes in experimental group 4. After 6 minutes of dynamic burdens, all were returned to the status of static compression for another 30 minutes and then electrical examination was made. Results After 2 hours of compression, motor and sensory nerve conduction velocity (NCV) of all the four experimental groups decreased significantly (P<0.05), but there was no significant difference between them. There was no significant change in the control group. There was no significant change of NCV in experimental group 1 during the last 30 minutes of experiment. NCV in the other three experimental groups decreased after introduction of dynamic burdens, especially in the experimental group 3. Conclusion The above results showed that NCV of cauda equina decreased significantly under dynamic burdens during static compression. Two kinds of dynamic burdens introduced at the same time can cause more profound change than a single one.更多还原