【摘要】 目的　探讨自体骨 -前交叉韧带 (ACL ) -骨、同种异体骨 - ACL -骨移植的形态学、组织学及超微结构的变化特点。　方法　将日本大耳白兔和新西兰兔各 6 0只随机分成自体骨 - ACL -骨移植组和二步冷冻保存同种异体骨 -ACL-骨移植组 ,每组分别有两种兔各 30只 ,对照组为自体正常侧 ACL。术中及术后均不使用免疫抑制剂。术后 4、12周分别切取 ACL 作大体、组织学和电镜检测。　结果　自体移植组重塑型速率要快于二步冷冻保存同种异体骨 - ACL-骨移植组。 4周时自体移植组和冷冻异体移植组前交叉韧带的胶原纤维中大直径纤维 (≥ 80 nm)分别为 6 %、2 4 % ,小直径纤维 (<80 nm )分别为 94 %、76 %。 12周时前交叉韧带的胶原纤维中大直径纤维 (≥ 80 nm)分别为 0 %、2 % ,小直径纤维(<80 nm)分别为 10 0 %、98% ,电镜下可见自体、冷冻异体移植组重塑过程均相似。光镜下两者有相似的组织学愈合过程。　结论　自体骨 - ACL-骨移植和二步冷冻保存同种异体骨 - ACL-骨移植 ,具有相似重塑型过程和组织学愈合过程 ,自体移植组重塑形速率要快于二步冷冻保存同种异体骨 - ACL-骨移植组。更多还原

【Abstract】 Objective To study the characteristics of, morphology histology and ultrastructure of anterior cruciate ligament(ACL) autograft and two step cryopreserved ACL allograft after transplantation. Methods Sixty New Zealand rabbits and sixty Japanese rabbits were randomly divided into two groups :ACL autograft group and two step cryopreserved ACL allograft group .Immunosuppressant were not used after transplantation. The histology and ultrastructure of the ACL of transplantation and normal knee were observed after 4 weeks and 12 weeks, respectively. Results The rate of remodeling process was faster in ACL autograft than in two step cryopreserved ACL allograft, but there was similar remodeling process between two groups 12 weeks after transplantation. The proportions of large diameter fibers(≥80 nm) of ACL autogrft and cryopreserved ACL allograft were 6% and 24% in the 4th week, and were 0 and 2% in the 12th week, respectively. The proportions of small diameter of fibers(<80 nm) of ACL autogrft and cryopreserved ACL allograft were 94% and 76% in the 4th week, and 100% and 98% in the 12th week, respectively. Histologic incorporation in ACL autograft was similar to that in cryopreserved ACL allograft. Conclusion Two step cryopreserved bone ACL bone allograft were similar to bone ACL bone autograft cryopreserved in remodeling process and histology. The rate of remodeling process was faster in ACL autograft than in cryopreserved ACL allograft. [更多还原