【摘要】 目的　研究细胞周期在组织工程研究中的应用 ,为材料的生物相容性评价提供一种新思路。　方法　利用免疫细胞化学 ,蛋白质印迹法 ,RT- PCR和流式细胞术等方法分析细胞周期和对与细胞周期相关的基因表达进行研究。　结果　通过研究材料和细胞的相互作用 ,表明材料的拓扑结构、物理和化学结构都会影响吸附在材料上的细胞的细胞周期。并且会影响与细胞周期、增殖和凋亡相关基因的表达。　结论　细胞周期的研究能够综合反映出细胞增殖、分化及凋亡等生理活动的动态平衡 ,有希望成为生物相容性研究的一种重要方法。更多还原

【Abstract】 Objective To investigate cell cycle as a new tool to evaluate the biocompatibility of biomaterials.Methods The cell cycle and the expression of related genes were analyzed by the methods of immunocytochemistry, protein blotting, RT PCR and flow cytometry. Results The physical properteis, chemical properties and topological properities of biomaterials could not only influence cell cycle of the cells attached onto biomaterials but also affect the expression of related genes of target cells. Conclusion As an important extension of routine proliferation epxeriments, the study of cell cycle control will be great help for us to study the cell group as an organic society. It revealed the balance between cell proliferation, cell differentiation and apoptosis. It is suggested that the study of cell cycle control will play a key role in the research of tissue engineering.更多还原