【摘要】 利用适用于禾谷类作物的表达载体pGU4AGBar和pGBIU4AGBar,采用花粉管通道法 ,将人工合成的雪花莲凝集素基因sgna导入了优良冬小麦品系西农 2 2 0 8和西农 1 3 2 ,经PCR和Southernblot鉴定 ,证明获得了 2 0株导入了sgna基因的转基因植株 ,转化率约为 0 .2 8%～ 0 .84% ,并通过Westernblot鉴定检测到了目的蛋白的表达更多还原

【Abstract】 In the report, the gramineous expression vectors pGU4AGBar and pGBIU4AGBar were used, respectively. The s gna gene, a synthetical agglutinin gene of Galanthus navies , had been transferred into the winter wheat varieties Xinong 2208 and Xinong 132 by pollen_tube pathway. The PCR and Southern blotting analysis showed that 20 transgenic plants with s gna gene were obtained. Western blotting analysis revealed that the target protein was expressed in the transgenic plants. The transformation frequency were 0.28%～0.84%.更多还原