【摘要】 目的探讨黏附分子与树突状细胞 (DC)在大鼠单侧输尿管梗阻后肾组织中的表达和分布 ,以及抗P -选择素单抗的抗黏附调抑作用。方法建立大鼠单侧输尿管梗阻 (UUO)模型 ,随机将大鼠分为手术组 (n=18)和抗P -选择素单抗治疗组 (n=18) ,按不同梗阻时间 (1、3、7d)再分为3组 ,另设假手术组 (n=6)作为对照。分别采用免疫组化LSAB法和免疫双染与荧光图像分析法 ,观察P -选择素、ICAM -1及DC在肾组织中表达和分布变化。结果大鼠UUO第1d起即见P -选择素以肾小管上皮细胞为主在肾小管间质中表达上调 ,伴随CD1a+ CD80+ DC在以肾间质为主部位的浸润。第3dP -选择素明显表达 ,ICAM -1以肾血管为主表达上调 ,且CD1a+ CD80+ DC显著增多。至第7dP -选择素表达遂下调 ,ICAM -1表达明显增强 ,而CD1a+ CD80+ DC在肾间质分布聚集达峰。经抗P -选择素单抗处理后 ,大鼠肾组织P -选择素、ICAM -1表达受到抑制 ,CD1a+ CD80+ DC分布聚集减少 ,且肾小管间质病理损伤减轻。结论P-选择素等黏附分子介导树突状细胞迁移聚集 ,可能参与了肾小管间质纤维化早期病理损伤过程 ,抗P -选择素单抗对此具有抗黏附调抑和肾脏保护作用。更多还原

【Abstract】 Objective To explore the expression of the adhesive molecules and distribution of dendritic cells(DCs)in rat kidney with unilateral ureteral obstruction as well as the preventive effect of anti-P-selectin lectin-EGF domain monoclonal antibody(PsL-EGFmAb).Methods Rat models of unilateral ureteral obstruction were established,which were divided into sham group(n=6)、untreated group(n=18)and treated group with PsL-EGFmAb(n=18).DCs were analyzed with Axioplan2microscopy,while P-selectin and ICAM-1were analysed by immunohistochemistry LSAB,the change of renal pathology was observed at the same time.Results P-selectin was present in renal tubular epithelial cell after unilateral ureteral obstruction on day1,and CD1a + CD80 + DC was also found in renal interstitium;P-selectin and DCs presented were increased evidently on the3 rd day,and the expression of ICAM-1was up-regulated in renal vessel.The expression of P-selectin was deˉcreased,but ICAM-1was increased and the accumulation of DCs was the most in renal interstitium on the7 th day.These changes became less conspicuous in rat treated with PsL-EGFmAb.Conclusions DCs immigration and accumulation may play an important role in early tubulointerstitial lesions.PsL-EGFmAb has anti-adhesive effect on it.更多还原