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小麦类核糖核酸酶基因(WRN1)cDNA在自然衰老和黑暗诱导衰老条件下的表达(英文)
Expression of a Wheat S-like RNase (WRN1) cDNA During Natural-and Dark-induced Senescence
【摘要】 从普通小麦(Triticum aestivum L.)中分离了一个类核糖核酸酶(WRN1)基因的cDNA。WRN1的转录受自然衰老和黑暗诱导衰老的负调控。在幼嫩组织中WRN1也有表达。由于在两个保守的位置上组氨酸被替换,WRN1很可能已经失去了核糖核酸酶的活性。 Southern分析表明,在普通小麦基因组中,WRN1以一个小基因家族的形式存在。
【Abstract】 An S-like RNase cDNA had been isolated from common wheat (Triticum aestivum L.). Thetranscription of WRN1 mRNA was down-regulated by natural- and dark-induced senescence. But it was notsenile-tissue-specific. As the two key histidine residues were replaced, WRN1 may not be active as RNase.Southern blotting analysis showed that WRN1 exists as one of a small gene family in common wheatgenome.
【关键词】 小麦类核糖核酸酶(WRN1);
叶片衰老;
普通小麦;
【Key words】 wheat S-like RNase (WRN1); leaf senescence; common wheat;
【Key words】 wheat S-like RNase (WRN1); leaf senescence; common wheat;
【基金】 国家重点基础研究发展规划项目(G1998010200)。~~
- 【文献出处】 Acta Botanica Sinica ,植物学报(英文版) , 编辑部邮箱 ,2003年09期
- 【分类号】S512.101
- 【被引频次】11
- 【下载频次】158