【摘要】 当蓝细菌Synechocystissp .PCC 6 80 3在添加葡萄糖的BG₁1培养基中培养时 ,细胞出现了一种新脂。这种脂经浓硫酸 /α_萘酚染色反应证实为糖脂 ,记为糖脂_x。这一糖脂的出现伴随着其他脂、尤其是双半乳糖甘油二酯含量的下降。此外 ,在添加果糖、麦芽糖、乳糖等其他碳源的培养基中生长的细胞中也检测到这一糖脂。活性氧猝灭剂硫代硫酸钠加入到培养基中能有效地抑制糖脂x的出现。当在BG₁1培养基中加入 0 .3%硫代硫酸钠后 ,糖脂x仅能在培养基中添加高浓度 （10 0mmol/L）的葡萄糖且细胞生长处于后指数期时检测到。这些结果表明蓝细菌Synechocystissp .PCC 6 80 3细胞在含葡萄糖的培养基中生长出现的一种新糖脂可能与活性氧有关更多还原

【Abstract】 A novel lipid occurred when cyanobacterium Synechocystis sp. PCC 6803 cells were grown in BG₁1 medium with glucose applied. This lipid was determined to be a glycolipid, designated glycolipid_x （Glyco_x）, by staining with α_naphthol and concentrated sulfuric acid. The occurrence of Glyco_x accompanies the disappearance of other lipids, especially DGDG. Glyco_x can also be observed in cells grown in BG₁1 medium with the application of other carbon sources: fructose, maltose and lactose. Sodium thiosulfate, an effective scavenger of reactive oxygen intermediates, showed strong capability to inhibit glucose_induced occurrence of Glyco_x. In the presence of 0.3% sodium thiosulfate, Glyco_x could only be detected in cells grown in BG₁1 medium with 100 mmol/L glucose applied in late_exponential phase. These results suggest that reactive oxygen species might be involved in the occurrence of Glyco_x in cyanobacterium Synechocystis sp. PCC 6803 cells grown in the presence of glucose.更多还原