【摘要】 采用RT_PCR差异显示法 ,从水稻 (OryzasativaL .)幼苗克隆了 1个受冷抑制表达的cDNA片段。该片段序列与水稻叶绿体基因组编码ATP合酶CF0 Ⅲ亚基的atpH基因完全同源 ,且覆盖了atpH基因编码区。以Northern杂交分析了水稻幼苗在冷处理不同时间后的atpH基因转录水平 ,结果表明 ,atpH基因的转录受冷抑制 ,在冷处理第 1天就明显下降 ,第 2天以后完全受抑制。更多还原

【Abstract】 A chilling-repressed cDNA fragment was cloned from rice (Oryza sativa L.) seedlings by reverse transcription-polymerase chain reaction (RT-PCR) differential display. Database comparison of the cDNA sequence is identical to atpH encoded by rice chloroplast genome. Northern hybridization showed that its RNA transcript was reduced greatly on the first day and fully inhibited after 2 d of chilling treatment.更多还原