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豌豆根胞质V₁-ATPase的鉴定（英文）

Characterization of Cytosolic V₁-ATPase in Pea Root

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【Author】 YU He_Fen 1,2 , CHEN Jia 1 *, WANG Xue_Chen 1 (1. College of Biological Sciences, China Agricultural University, State Key Laboratory of Plant Physiology and Biochemistry, Beijing 100094, China; 2. National Laboratory of Biomacromolecules, Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China)

【机构】中国农业大学生物学院植物生理生化国家重点实验室；中国农业大学生物学院植物生理生化国家重点实验室北京100094中国科学院生物物理研究所生物大分子国家重点实验室；北京100101；北京100094；北京100094；

【摘要】利用免疫印迹、免疫电镜和ATP水解活性的测定对豌豆 (PisumsativumL .)根细胞胞质中V1_ATPase复合物的存在进行鉴定。用兔抗绿豆V_typeH+ _ATPase的A、B亚基的抗体进行的immuno_blotting和胶体金电镜结果都表明 ,胞质中存在有A、B亚基。活性测定结果进一步表明胞质具有ATP水解活性。这些结果说明豌豆根胞质具有有活性的V1_ATPase复合物。这是首次直接证明植物中有胞质V1_ATPase的存在。更多还原

【Abstract】 Cytosolic V 1_ATPase complex in pea ( Pisum sativum L.) root cells was characterized by immuno_blotting, immuno_electron microscopy and measurement of ATP hydrolysis activity. Using antibodies against the subunits A and B of V_type H +_ATPase (V_ATPase) from mung bean as primary antibodies, both immuno_blotting and immuno_electron microscopy showed that subunit A and subunit B existed in the cytosol. Measurement of ATPase activity further showed that cytosolic proteins had NO - 3_sensitive ATP hydrolysis activity. All these suggested that V 1_ATPase complex occurred in the cytosol. This is the direct proof that V 1_ATPase occurs in the cytosol of the plant cells for the first time.更多还原