【摘要】 对山东省侵染马铃薯的一个马铃薯X病毒（PVX）分离物PVX-SD₁的外壳蛋白（CP）基因进行了克隆和序列分析。以提纯的病毒RNA为模板,应用RTPCR扩增目的基因,通过常规的基因克隆法将扩增的CP基因导入pUC19载体,测序。结果表明,PVX-SD₁的CP基因长719bp,可编码248个氨基酸;与Gen-Bank中报道的15个有代表性的株系或分离物相比较,核苷酸同源性在80.1％～99.7％,氨基酸同源性在89.8％～100％;与欧洲株系UK₃仅1个核苷酸不同,同源性为99.7％,氨基酸同源性达100％,表明它们可能为同一株系,属于X³细。更多还原

【Abstract】 An isolate of potato virus X ( PVX-SD;) was obtained from potato plants cultivated in Shandong Province. The coat protein ( CP) gene of PVX was amplified from the extracted viral RNA by using reverse transcription -poly merase chain reaction (RT-PCR) , and cloned into plasmid pUC19. The nucleotide sequence of the CP gene was determined and compared with other isolates reported in GenBank. It shares 80. 1% -99. 7% and 89. 8% - 100% homology with the reported isolates at the sequence levels of nucleotide and deduced amino acid, respectively. PVX-SD1 shares 99. 7% and 100% homologies at the nucleotide and deduced amino acid levels, respectively, with one of the European strain UK3, indicating that they could belong to the same strain.更多还原