【摘要】 探讨了以减毒鼠伤寒沙门氏菌为载体传递新城疫病毒 DNA疫苗的安全性、免疫原性和可行性。将含新城疫病毒 ( NDV) F4 8E9株融合蛋白 ( F)基因的真核表达质粒 pc DNA3- F的重组减毒鼠伤寒沙门氏菌 ZJ111株 ( ZJ111/ pc D-NA3- F菌株 ) ,以 10 8CFU进行首免 ,2周后二免 ,二免后 4周攻击强毒株 F4 8E9,观察其安全性和免疫原性 ,同时设只含空载体 pc DNA3的 ZJ111/ pc DNA3菌株对照及口服 PBS对照。结果表明 :重组 ZJ111/ pc DNA3- F菌株具有良好的安全性 ,对强毒株攻击的保护率达 6 4 .7%。重组 ZJ111/ pc DNA3- F菌株不仅能诱导雏鸡产生 NDV EL ISA抗体 ,而且诱导产生的法氏囊 B淋巴细胞和胸腺 T淋巴细胞增殖反应显著高于 ZJ111/ pc DNA3对照组。这些结果提示 ,减毒沙门氏菌为载体不仅可直接将 NDV F基因呈递给鸡体细胞进行表达 ,产生抗 NDV的体液免疫 ,而且还可诱导细胞免疫应答。更多还原

【Abstract】 The safety and immunogenicity of Newcastle disease virus(NDV) DNA vaccine using attenuated Salmonella ty phimurium as carrier were reported here.The recombinant attenuated Salmonella typhimurium strain ZJ111(dam - and phoP -) carried pcDNA3 F expression plasmid encoding the fusion protein of NDV(ZJ111/pcDNA3 F).Three day old nonimmunized chickens were divided into 3 groups:the first group immunized orally with ZJ111/pcDNA3 F,and the second group with ZJ111/pcDNA3.The last group was immunized orally with PBS as control.All these groups were boosted two weeks later.At week 4 post boosting immunization,all groups were challenged with a lethal dose of NDV F 48 E 9 strain.The results showed that the ZJ111/pcDNA3 F was safe for chickens,and 64 7% of chickens were protected from the challenge.Specific antibody as well as T and B cell responses were induced by the DNA vaccine.This study clearly indicted that the attenuated Salmonella typhimurium could deliver the NDV F gene for expression within the chicken body and induce both the humoral and cellular immunity.更多还原