【摘要】 环氧合酶 (cyclooxygenase,Cox)是由花生四烯酸合成前列腺素的限速酶 ,而前列腺素在哺乳动物的生殖过程中起着很重要的作用。本试验采用 RT- PCR方法对环氧合酶 - 1(Cox- 1)及环氧合酶 - 2 (Cox- 2 )在早期妊娠兔子宫内膜中的表达进行了半定量研究和在早期胚胎中的表达定性研究。结果表明 ,Cox- 1在早期妊娠兔子宫内膜中表达较平稳 ,呈组成型表达 ;在早期胚胎中各个时期均可以检测到 Cox- 1m RNA,表明它在早期胚胎发育过程中起作用。 Cox- 2在早期妊娠子宫内膜中的表达水平有很大差异 ,妊娠第 1天表达水平较高 ,从第 2天开始表达下降 ,到第 6天表达又明显增加 ,第 8天达到最高。 Cox- 2在囊胚之前的各个时期胚胎均未检测到 ,而在扩展胚泡和孵出胚泡中表达 ,Cox- 2的时期特异性表达可能与兔胚泡孵出及胚胎着床等过程相关。更多还原

【Abstract】 Cyclooxygenase is a rate limiting enzyme that converts arachidonic acid to prostaglandin.It exists as two isoforms:cyclooxygenase 1(Cox 1) and cyclooxygenase 2(Cox 2),which are encoded by separate genes.RT PCR method is used to study the expression of Cox 1 and Cox 2 in rabbit endometrium during early pregnancy by semi quantitative method and to study their expression in early stage embryos by qualitative method.Cox 1 is constitutively expressed in the endometrium during early pregnancy and it can be detected in every stage of early embryos.There is obvious change for Cox 2 expression in the endometrium.A high level of Cox 2 expression is detected on day 1 of pregnancy,it decreases from day 2 to 5,increases again from day 6,and reaches its highest level on day 8.Cox 2 can only be detected in expand blastocyst and hatched blastocyst.The stage specific expression of Cox 2 may be related to embryo hatching and implantation.更多还原