【摘要】 从海水网箱养殖的患病军曹鱼 (Rachycentron canadum)的肾脏、肝脏等实质器官及腹水中分离出 1株致病菌即JA- 4 6菌。其特点如下 :革兰氏阴性短杆菌 ,极端单鞭毛 ,大小为 (1～ 2 )μm× (0 .5～ 1)μm,氧化酶和过氧化氢酶阳性 ,有运动性 ,最适温度 2 8～ 30℃ ,需盐生长 ,未见发光 ,对弧菌抑制剂 0 / 12 9敏感 (15 0 mg/ L) ,发酵葡萄糖不产气 ,在TCBS上菌落呈黄色。经 API- 2 0 E系统试剂盒检测和 ATB自动鉴定系统鉴定 ,该菌为创伤弧菌 (Vibrio vulnificus) ;其半数致死量 (L D50 )为 4 .1× 10 4 个细菌。药敏试验结果表明 ,氯霉素、复方新诺明、庆大霉素、环丙沙星、红霉素、氟哌酸、先锋霉素和丁胺卡那霉素等 8种药物对该菌株有抑制作用 ,但该菌株对青霉素和氨苄青霉素不敏感更多还原

【Abstract】 A pathogenic JA 46 strain was isolated from kidney ,liver and ascites of moribund fish.The bacterium shared following characteristics:Gram negative,short rod,single polar flagellum,motile,(1 2)μm×(0 5 1)μm in size,oxidase and catalase positive,sensitive to the vibriostatic agent O/129 at 150 mg/L,fermentation of glucose without production of gas,growth only in medium contained NaCl,optimal temperature of 25 30℃,no luminescence,yellow colonies in TCBS medium.The JA 46 strain was identified as Vibrio vulnificus by the data of API 20E kit and ATB system.The 50% lethal dose of the bacterium was 4 1×10 4 cpf/mL.The data of drug sensitivities indicated that chloramphenicol,sulfamethoxazole,gentamycin,erythromycin,ciprofloxacin,norfloxacin,cefalexin and amikacin could inhibit growth of JA 46 strain,but penicillin and ampicillin didn′t.更多还原