【摘要】 从山东、江苏、上海等地的鸡场中 ,收集疑为网状内皮组织增殖病病毒 (REV)感染鸡的脾脏、肝脏 5 6份 ,分别通过聚合酶链式反应 (PCR)、班点杂交试验 (Dot- blot)和间接免疫荧光试验 (IFA)对其进行 REV检测。结果有 2 1份呈PCR阳性 ,2 0份呈 Dot- blot阳性 ,15份呈 IFA阳性 ,且所有 IFA阳性样品 ,PCR和 Dot- blot检测都呈阳性 ,2 0份Dot- blot阳性样品中有 19份呈 PCR阳性。研究表明 ,PCR检测 REV较其他方法敏感 ,可作为 REV的常规检测方法之一。更多还原

【Abstract】 Fifty six samples of spleen and liver of flock suspected infected by reticuloendotheliosis virus(REV) were collected from Shangdong,Jiangsu and Shanghai.Polymerase chain resction(PCR),Dot-blot and IFA were used to inspect the presence of REV.The results show the positive samples are 21,20 and 15 respectively in the tests of PCR,Dot-blot and IFA;All the positive samples in IFA are also positive in PCR and Dot-blot;There are 19 PCR positive samples in all the 20 Dot-blot positives.In this study,PCR was more sensitive than Dot-blot and IFA,and it is a preferable assay to inspect the infection of REV.更多还原