【摘要】 [目的]通过视神经外伤后视网膜形态的变化,了解外伤性视神经损伤的手术时机与疗效间的相关关系。[方法]建立外伤性视神经损伤和不同时间减压动物模型,对视网膜神经节细胞(RGCs)、视网膜等进行了定量分析。[结果]正常对照组RGCs相对体积数密度(体密度,V_v)为0.072,小细胞所占比例为49.5％,视网膜厚度为108.9μm,节细胞以内层的厚度为19.5μm;48h减压组RGCs体密度为0.052,小细胞占比例为66.8％,视网膜总厚度为101.9μm,节细胞以内层厚度为16.9μm;14d减压组RGCs体密度为0.042,小细胞占比例为76.4％,视网膜总厚度为96.5μm,节细胞以内层为15.5μm。[结论]48h减压较14d减压可以较好的保存RGCs和视网膜的形态,外伤性视神经损伤后应该尽早解除周围因素对视神经的压迫。更多还原

【Abstract】 [Objective] Through observation of retinal change, to understand the relationship between the operative time and effectiveness traumatic optic neuropathy. [Method] Animal model (Guinea pig) of traumatic optic neuropathy and nerve decompressing at different time were established, followed by quantitative study of retinal structure and retinal ganglion cells (RGCs). [Results] RGCs relative volume density (relative number of cumulus RGCs populations) in normal Guinea pig was 0. 072 (small RGCs 49. 5% ). The complete retina thickness was 108. 9μm and RGCs layer was 19. 5μm. 48 h nerve decompression group; RGCs relative volume density was 0. 052 ( small RGCs 66. 8% ), and the complete retina thickness was 101. 9μm and RGCs layer was 16. 9μm. 14 d nerve decompression group: the complete retina thickness was 0. 042 ( small RGCs 76. 4% ), and the complete retina thickness were 96. 5μm and RGCs was 15. 5μm. [Conclution] The shape of RGCs and retina is better preserved in 48 nerve decompression than in 14 d group. The optic nerve decompression should be done as soon as possible.更多还原