【摘要】 [目的]探讨汉防已碱(Tet)对内毒素诱导葡萄膜炎的预防和治疗作用。[方法]将200μg伤寒杆菌内毒素(LPS)注射于20只SD大鼠足底部,诱导出葡萄膜炎(EIU)模型,其中12只大鼠于LPS注射前半小时开始给于汉防已碱40mg灌服,共4次,每隔4h一次。另外8只未给予灌服作为对照。于 LPS注射后6 h、12 h、24 h用裂隙灯显微观察其临床改变,并于LPS注射后24 h处死大鼠,观察各大鼠眼部组织学改变。[结果]LPS注射后均诱发出双眼相似的葡萄膜炎,炎症的严重程度在未处理组临床积分为7.65±0.67,Tet组临床积分为3.27±1.02,P<0.01,有显著差异。组织学检查,在Tet处理组大鼠,睫状体、脉络膜、视网膜及玻璃体腔炎症细胞浸润均比未处理组明显减轻。[结论]Tet对内毒素诱发的葡萄膜炎有一定的抑制治疗作用。更多还原

【Abstract】 [Objective] To investigate the effect of tetrandrine (Tet) on endotoxin induced uveitis in rats. [Method] Injection of 200μg Salmonella typhimurium lipopolysaccharide (LPS) into hind footpad was performed in 20 Spar-Dawley rats to induce uveitis model. Of which 12 rats were treated with 40 mg Tet by administration (p. o. ) for 4 times from 0.5 hour prior to LPS injection with an interval of 4 hours. The examination with slit-lamp microscopy was performed at 6, 12 and 24 hours after LPS injection. Pathological changes were evaluated at 24 hours after LPS injection. [Results] U-veitis was induced in all of the rats that received LPS injection. Uveitis was significantly less severe in the Tet-treated rats (score: 3.27±1.02) as compared with that in the controls(score: 7.65 ±0.67). Infiltration of inflammatory cells and exudation in Tet-treated SD rats were also significantly reduced as compared with those in the untreated SD rats. [Conclusion] Tet is effective in reducing the intraocular inflammation induced by endotoxin.更多还原