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活性乳酸菌制品中乳酸菌计数培养条件探讨

A method of lactic acid bacteria counting in living lactic acid bacteria products

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【作者】 张华丽齐晋莲梁艳孙静龙霞隋凌云苏敬武

【Author】 Zhang Huali,et al. (Health and Anti-epidemic station of Rongcheng city, Rongcheng Shandong\ 264300,China)

【机构】 荣成市卫生防疫站威海市疾病控制中心 山东荣成264300山东荣成264300山东威海264200

【摘要】 为快速、准确地计数活性乳酸菌制品中的乳酸菌 ,用CO2 烛缸法代替现行国家标准GB T16 347— 1996活性乳酸菌饮料中乳酸菌微生物检验方法中的有氧培养法。CO2 烛缸法操作简便 ,计数准确可靠。在菌种对比实验中 ,CO2 烛缸法的乳酸菌计数结果是国家标准方法的 8倍。在试样的对比实验中 ,CO2 烛缸法的乳酸菌计数结果是国家标准方法的 2 0倍。CO2 烛缸法较目前国家标准方法更能真实地反映活性乳酸菌制品中乳酸菌的数量。

【Abstract】 In order to count lactic acid bacteria in living lactic acid bacteria products accurately, we substituted the aerobic culture method adopted in present national standard in China with CO 2 method ( removing oxygen from an airtight container by burning of candle). In bacterial strain comparison experiments, the counting result of lactic acid bacteria by CO 2 method was 8 times more than that by aerobic culture method. In sample comparison experiments, the counting result of lactic acid bacteria by CO 2 method was 20 times more than that by aerobic culture method. The result suggested that the CO 2 method better than aerobic culture method with its simplicity, rapidity and accuracy.

  • 【文献出处】 中国食品卫生杂志 ,Chinese Journal of Food Hygiene , 编辑部邮箱 ,2003年01期
  • 【分类号】R155.5
  • 【被引频次】14
  • 【下载频次】431
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