【摘要】 RNA干扰 (RNAi)是指生物体内由双链RNA介导同源序列mRNA的特异性降解 ,从而导致基因沉默的现象。近年来 ,随着对RNAi研究的不断深入 ,其作用机制正在逐步被阐明 ;同时作为阻断基因表达的新手段 ,RNAi技术也日趋完善和成熟。以其高效性和特异性 ,RNAi为反向遗传学在神经科学研究中的应用提供了有力的工具。本文仅就RNAi技术的有关研究进展及其在神经科学研究中的应用作一概述。更多还原

【Abstract】 RNA interference (RNAi) is a way by which double-stranded RNA degrades its homological mRNA and leads to gene silencing in cells. With the further studies of RNAi in the last few years, more about its mechanisms has been elucidated. Moreover, as a technique to inhibit gene expression, RNAi is becoming more and more perfect in the studies of functional genomics. It also provides a powerful tool for reverse genetics in neurscience research. The recent advancement in RNAi technique and its application in neuroscience research were concisely reviewed in this paper.更多还原