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饲料铜的添加量对南美白对虾生长、血液免疫因子及组织铜的影响

Effects of dietary copper on growth,immunological factors and tissue copper in Litopeneaus vannamei

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【作者】 郭志勋陈毕生徐力文郑石轩湛波刘立鹤程开敏

【Author】 GUO Zhixun1, CHEN Bisheng1, XU Liwen1,DENG Shixuan2, ZHAN Bo2, LIU Lihe2, CHENG Kaimin2(1.South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300 China;2.Zhanjiang Yuehai Feed Company, LTD., Zhanjiang 524002 , China)

【机构】 中国水产科学研究院南海水产研究所农业部渔业生态环境重点开放实验室广东省渔业生态环境重点实验室湛江粤海饲料有限公司湛江粤海饲料有限公司 广东广州 510300广东广州 510300广东湛江 524002广东湛江 524002

【摘要】 通过在饲料中分别加入不同水平的硫酸铜,初步研究了饲料铜的添加量与南美白对虾(Litopenaeusvannamei)生长、血液免疫因子及组织中铜分布的关系。饲料铜元素的添加量分别为0、7.5、15、30、45mg/kg。研究结果表明,铜的含量对南美白对虾生长影响显著,添加水平30mg/kg的实验组生长速度显著高于其他组(P<0.05),成活率各组间差异不显著。实验组对虾的酚氧化酶(PO)和超氧化物歧化酶(SOD)的活力都高于对照组,其中当添加水平为30mg/kg时,两种酶的活力均最强。对虾肌肉铜的含量各组间无显著差异,为(4.27±1.83)mg/kg,个体间差异非常大;肝胰脏(包括淋巴器官)铜的最高含量为210.36mg/kg,最低为33.78mg/kg(空白对照组),明显高于肌肉中的含量。研究结果表明,饲料铜的含量对对虾生长影响显著,并影响对虾的免疫能力;肝胰脏是对虾体内铜的主要积累器官。

【Abstract】 The designed levels of Cu2+ in diets were 0, 7.5, 15, 30 and 45 mg/kg, respectively. The experiment period was two months. The shrimp fed with the feed with Cu2+ 30 mg/kg grew faster than the other groups significantly. But the differences in survival rates were not significant. The activities of PO and SOD were influenced by the content of feed Cu2+ that they were highest when the Cu2+ level was 30 mg/kg feed. There were not significant differences in shrimp muscle copper content among different groups, of which the average value was (4.27±1.83) mg/kg, but it varied obviously among the individuals. The highest content of the hepatopanreas Cu2+ was 210.36 mg/kg, and the lowest was 33.78 mg/kg (control group), which was higher than that in the muscle. The results showed that the dietary copper level has effects on growth rate and immunological function in Litoenaeus vannamei; the accumulation of copper in shrimp hepatopancreas is higher than that in muscle. The optimal dietary copper level is 30 mg/kg.

【关键词】 南美白对虾生化指标
【Key words】 copperLitoenaeus vannameibiochemical index
  • 【文献出处】 中国水产科学 ,Journal of Fishery Sciences of China , 编辑部邮箱 ,2003年06期
  • 【分类号】S963
  • 【被引频次】35
  • 【下载频次】559
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