【摘要】 取体重约300g鲜活鲫(Carassiusauratus)的肝组织进行总RNA提取。从GenBank数据库查询已发表的8种鱼转铁蛋白cDNA或基因序列,根据铁离子结合转运功能位点,设计并合成了2对引物P1、P4以及P2、P3,克隆出鲫血清转铁蛋白cDNA中的从大约550bp至1450bp的核心片段,长度为0.9kb。同时,比较了几种鱼血清转铁蛋白cDNA序列的同源性,并推导出鲫血清转铁蛋白cDNA核心片段的氨基酸序列,并分析了该氨基酸序列的结构和特性。更多还原

【Abstract】 The total RNA was extracted from the liver tissues of Carassius auratus. Two pairs of primers P1,P4 and P2,P3 were designed and synthesized according to the banding sites of iron while referring to the published cDNA or gene sequences of fish transferrins (Tf)in the Database of GenBank. A key segment sequence ( 0.9 kb in length) of Crucian carp serum transferrin cDNA was cloned from 550 bp to 1 450 bp. The comparison of gene or cDNA sequence identity in serum transferrins between several fishes was researched and the results showed that the identity of Tf gene or cDNA between C.auratus and most sea water fishes , such as Oryzias latipes, Oncorhynchus kisutch, O.tshawytscha, Platichthys flesus, Paralichthys olivaceus, P. olivaceus, Melanogrammus aeglefinus, was at 40%-50%. The amino acid sequence of the key segment sequence was deduced and the structure and characteristics of the amino acid sequence were analyzed also.更多还原