【摘要】 目的　研究人胚肺 (HEL)细胞HOXB基因的表达状态及人类巨细胞病毒 (HCMV)感染对HEL细胞HOXB基因表达的影响 ,以探讨HCMV致畸的分子机制。方法　采用半定量逆转录 -聚合酶链反应 (RT -PCR)技术。结果　①HEL细胞表达HOXB2 ,HOXB3,HOXB4 ,HOXB5 ,HOXB6 ,HOXB7和HOXB8,但不表达HOXB1和HOXB9;②HCMV感染后 ,HEL细胞被诱导表达HOXB9;且HOXB2 ,HOXB6 ,HOXB7和HOXB8基因的表达上调 ,而HOXB3,HOXB4和HOXB5基因的表达在HCMV感染后不同时间无明显变化 ,HOXB1基因仍旧不表达 ;结论　HCMV能诱导HOXB2 ,HOXB6 ,HOXB7,HOXB8和HOXB9基因表达异常 ,这可能在先天性HCMV感染导致胚胎发育畸形中起重要作用更多还原

【Abstract】 Aim To study the expression of HOXB genes in human embryo lung (HEL) cells and the influence of human cytomegalovirus (HCMV) infection on the expression of these genes,which may demonstrate the molecular mechanism of abnormal embryogenesis induced by HCMV Method The expression of HOXB genes were detected with semi-quantitative RT-PCR method Results ① HEL cells expressed HOXB2,HOXB3,HOXB4,HOXB5,HOXB6,HOXB7 and HOXB8 genes,but they did not express HOXB1 and HOXB9 gene ②After HCMV infection,HEL cell were induced to express HOXB9 gene,and the expression of HOXB2,HOXB6,HOXB7,HOXB8 gene were elevated The expression of HOXB3,HOXB4,HOXB5 gene had no significant change HOXB1 still was not expressed Conclusion HCMV can induce the abnormal expressions of HOXB2,HOXB6,HOXB7,HOXB8 and HOXB9 genes,which may play an important role in HCMV induced abnormal embryogenesis更多还原