【摘要】 目的:建立重庆地区HBV流行株PreS/S、EnhII/CP/PreC基因标准参照序列,为HBV变异的研究奠定基础。方法:测定15例无症状携带者HBV的PreS/S、EnhII/CP/PreC序列,应用同源性分析和对齐比较等方法确定基因型/血清型,并拟定标准参照序列。结果:9株基因型B/血清型;adw2、3株基因型B/M清型ayw1、3株基因型C/血清型adrq+;建立的重庆地区HBV流行株(基因型B/血清型adw2)PreS/S、EnhII/CP/PreC标准参照序列与华东华南地区同亚型的标准参照序列比较分别有6个、1个核苷酸差异。结论:初步建立了重庆地区HBV流行株PreS/S、EnhIICP/PreC基因标准参照序列。更多还原

【Abstract】 Objective :To establish the consensus sequence of PreS/S and Enhll/CP/PreC in Chongqing,China. Methods :The genes of PreS/S and Enhll/CP/PreC of HBV in 15 AsC were sequenced. The genotype and serotype of HBV were determined. The HBV strain prevailing in Chongqing was found and its consensus sequence was established. Results: Nine strains were genotype B/serolype adw2, 3 were genotype B/serotype aywl and 3 were genotype B/serotype adrq+ . There were 6 and 1 site differences between the consensus sequences of PreS/S and Enhll/CP/PreC of HBV with genotype B/serotype adw2 and that in Southeast China. Conclu-sions:The consensus sequence of PreS/S and Enhll/CP/PreC of hepatitis B virus prevailing in Chongqing was established.更多还原