【摘要】 目的　探讨整合蛋白α5β1和微血管密度在胃癌组织中表达的临床意义及其相互关系。方法　采用免疫组化SP法检测了 3 5例胃癌组织及其中 10例胃癌淋巴结转移灶和 8例慢性浅表性胃炎组织中整合蛋白α5β1的表达及微血管密度 (MVD)。结果　胃癌组织中整合蛋白α5β1表达水平及MVD均显著高于慢性胃炎 (t=3 .3 2 ,P<0 .0 1;t =2 .3 0 ,P<0 .0 5 ) ;胃癌原发灶中整合蛋白α5β1的表达仅与胃癌浸润深度相关 (t =2 .2 9,P<0 .0 5 ) ,而MVD则与胃癌浸润深度、淋巴结有无转移及TNM分期之间密切相关 (t =3 .0 7,P<0 .0 1;t =2 .48,P<0 .0 5 ;t=2 .94,P<0 .0 1) ;胃癌淋巴结转移灶中整合蛋白α5β1表达水平较相应的原发灶显著增高 (t =2 .45 ,P<0 .0 5 ) ;整合蛋白α5β1的表达及MVD均与胃癌组织学分级无关(t=0 .15 ,P >0 .0 5 ;t=0 .41,P >0 .0 5 ) ;胃癌组织中整合蛋白α5β1不同表达水平间MVD的差异无显著性意义 (F =1.43 ,P >0 .0 5 ) ,相关性分析显示两者无显著相关性 (r =0 .15 6,P =0 .3 7)。结论　整合蛋白α5β1在胃癌组织中的表达显著上调并与胃癌的进展有关 ,有可能成为判断胃癌侵袭和转移能力的指标及基因治疗的靶标 ,其在胃癌血管生成中可能无重要作用更多还原

【Abstract】 Objective To investigate the clinicopathological significance of integrin α 5β 1 expression and microvessel density(MVD) in gastric cancer(GC) and the correlation of MVD with integrin α 5β 1. Methods The expression of integrin α 5β 1 was detected by means of immunohistochemical staining (SP method) on paraffin embeded tissue specimens from 35 primary gastric carcinoma(PGC), 10 metastasic lymph node of gastric cancer and 8 chronic superficial gastritis (CSG). Vascular endothelial cells were stained immunohistochemically using anti CD34 monoclonal antibody to recognize microvessel(MV) in 35 cases of PGC and 8 CSG, MV was counted in 4 hot spot per slide under light microscope (×400) and the average was defined as MVD. The results combined with clinicopathological parameters were analyzed statistically to characterize the role of integrin α 5β 1 and MVD in the progression of gastric cancer. Results Integrin α 5β 1 expression and MVD in PGC were significantly higher than those in CSG respectively ( t =3.32, P <0.01; t =2.30, P <0.05); the expression of integrin α 5β 1 in PGC showed only a correlation with the invasion depth of tumor ( t =2.29, P <0.05) while MVD showed all correlations with invasion depth,lymph node status and TNM stage ( t =3.07, P <0.01; t =2.48, P < 0.05 ; t =2.94, P <0.01). Neither integrin α 5β 1expression nor MVD showed a relation with differential of PGC ( t =0.15, P >0.05; t =0.41, P >0.05). Integrin α 5β 1 was significantly over expressed in lymph node metastatic cancer compared with that in corresponding PGC ( t =2.45, P <0.05); the difference of MVD showed no statistical significance among levels of integrin α 5β 1 expression in PGC ( F =1.43, P >0.05) and it showed no correlation with integrin α 5β 1 expression( r = 0.156, P =0.37).Conclusion Over expression of integrin α 5β 1 is present in GC and associates with the progression of tumor, implying that it may be viewed as the indicator of invasion and metastasis and the candidate target of gene therapy of gastric cancer. However, integrin α 5β 1 may not play an important role in the vascularization of GC.更多还原