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土壤芽孢杆菌NBT菌株理化诱变筛选及其对作物生长的影响

Mutagenesis of the Strain NBT of Bacillus edaphicus and Its Effect on Growth of Chili and Cotton

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【作者】 盛下放; 何琳燕; 陈珏;

【Author】 SHENG Xia fang, HE Lin yan, CHEN Jue (College of Life Science, Nanjing Agricultural University, Nanjing 210095)

【机构】 南京农业大学生命科学学院; 南京农业大学生命科学学院 南京210095; 南京210095; 南京210095;

【摘要】 用紫外线加氯化锂 (LiCl)复合诱变方式对释钾效能较强的土壤芽孢杆菌NBT菌株进行诱变处理 ,得到抗高渗 (2 %NaCl)突变株 32株。经高渗、高温及酸、碱诱导 ,突变株NBT 6和NBT 19能够在 10 %NaCl溶液中存活 ,同时能够耐受 35~ 5 5℃高温及pH 4~ 10的酸碱环境 ,两突变株的释钾活性与出发菌株相当 ,且传代稳定。辣椒、棉花盆栽试验表明 ,NBT 6和NBT 19不仅能在作物根际土壤增殖 ,而且能提高根际土壤有效磷、钾含量 ,改善植株的钾素营养 ,促进植株生长。

【Abstract】 A strain NBT capable of dissolving silicate minerals and promoting plant growth was treated with UV+LiCl, 32 high osmotic pressure resistant mutants(2% NaCl) were screened. Two mutants (NBT 6 and NBT 19 ) that could survive in the 10% of sodium chloride solution and resistant to high temperature (35-55℃) and acid base (pH 4-10 ) were obtained. In addition, the two mutants had the same ability as the starting strain (NBT) in dissolving potassium bearing minerals, and they were stable. Pot experiments for chili and cotton showed that the two mutants can propagate in rhizosphere, increase available P and K contents in rhizospheric soil, improve plant potassium nutrition, and promote plant growth.

【关键词】 土壤芽孢杆菌; 诱变; 辣椒; 棉花;
【Key words】 Bacillus edaphicus; Mutagenesis; Chili; Cotton;
  • 【文献出处】 中国农业科学 ,Scientia Agricultura Sinica , 编辑部邮箱 ,2003年04期
  • 【分类号】S182
  • 【被引频次】18
  • 【下载频次】273
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