【摘要】 用KOH DTT对小鼠单个卵子、 2 -cell胚、 8-cell胚、桑葚胚和囊胚进行处理 ,分别作为模板DNA ,并用PCR技术对其mtDNA的高变异区D -环区的 5′端 342bp片段进行扩增。结果 ,小鼠单个卵子、 2 -cell胚、 8-cell胚、桑葚胚或囊胚经KOH DTT处理后 ,紫外分光光度法测得DNA含量为 1 0～ 1 0 75mg ml,OD 2 6 0 OD 2 80值为 0 835～ 0 94 4;以这种DNA样品为模板 ,经一次PCR扩增即能获得清晰的目的DNA条带更多还原

【Abstract】 We have cultivated these cells from mice by KOH/DTT,such as single ovum,2-cell embryo,8-cell embryo,morula and blastocyst Then 5′ D-loop of mtDNA of these cells,which have 342 bp,have been amplification by PCR technology As a result,after these cells have cultivated by KOH/DTT,density of DNA,which has been tested by the mass UV-spectrophotometer,is 10～10 75 mg/ml and OD260/OD280 is 0 835～0 944 Acting these DNA as template DNA respectively,clear target DNA fragment could be attained by only one PCR amplification更多还原