【摘要】 目的 :探讨眼前房内注射牛视网膜可溶性抗原 (S抗原 )在诱导眼免疫耐受中的作用。方法 :自新鲜牛眼球中提取S抗原 ,注入Wistar大鼠眼前房内。 1周后 ,皮内注射牛S抗原与弗氏完全佐剂乳化混合物。观察大鼠实验性自身免疫性葡萄膜视网膜炎 (EAU)发作时间、临床表现及组织病理学改变 ;并以ELISA方法检测脾细胞培养上清液中的细胞因子。结果 :前房预处理组 (groupA)EAU的发生率明显低于阳性对照组 (groupB) ,所有具有阳性体征者均有组织病理学改变。前房预处理组动物脾细胞培养上清液中的IL 4浓度明显高于阳性对照组 ;而IFN γ水平明显低于阳性对照组。结论 :前房内注射牛S抗原可以诱导大鼠眼免疫耐受 ,在很大程度上抑制EAU的发生和发展。更多还原

【Abstract】 Objective:To study the effect of injection bovine retinal soluble antigen (S-Ag)into anterior chamber(AC)in inducing immunological tolerance.Methods:S-Ag was extracted from fresh bovine retina and inoculated into the AC of Wistar rats,One week later,the S-Ag mixed with complete fruend’s adjuvant(CFA)was injected into footpad intradermaly.The clinical symptoms and the pathological changes were observed.The IL-4 and IFN-γ concentrations in the supernatant of splenic cell culture were measured by ELISA.Results:The incidence of EAU in pretreated group(group A) was markedly higher than that of positive controls(gropu B).There were histopathological changes in all rats with clinical symptoms.The IL-4 level of splenic cell culture in group A were significantly higher than those in group B ( P<0.05 ).While IFN-γ level in group A were lower than those in group B (P<0 05).Conclusion:The occular immunological tolerance can be induced by AC injection bovine S-Ag,and inhibit the incidence and development of EAU.更多还原