【摘要】 目的 :研究Wee1转基因细胞抗CTL介导的细胞毒效应及抗穿孔素抗体对抗细胞毒效应的影响。方法 :体外混合NIT及淋巴细胞培养获得CTL ,并测定淋巴细胞增殖指数 (MTT法 ) ;采用脂质体将重组体Wee1Hu GFP导入哺乳动物细胞NIT ;以转染重组体前后的NIT为靶细胞 ,检测CTL介导的胞毒效应 (LDH法 ) ,同时测定抗穿孔素抗体对此效应的影响。结果 :混合细胞培养可诱导CTL的增殖 ;转染Wee1基因及加入抗穿孔素抗体NIT组发生的细胞溶解的数量最少。结论 :转Wee1基因靶细胞在一定程度上可抵抗CTL介导的细胞毒作用 ,穿孔素抗体可协同增强此抗胞毒作用。更多还原

【Abstract】 Objective:To study the effect of resistance to CTL mediated cytotoxity of target cells transfected with Wee1 and treated with anti perforin antibody Methods:Cytotoxic T lymphocyte was obtained by mixing lymphocyte NIT culture in vitro,and proliferation index (PI) of lymphocytes was detected(MTT method) The recombinant vector pCMV Wee1Hu/GFP was transfected into mammalian cells NIT With the function of anti perforin antibody, NIT and NIT transfected with Wee1 used as target cells, CTL mediated cytotoxity was detected by LDH method Results:CTL proliferation was induced by mixing cell culture With anti perforin antibody, the cytolysis quantity of NIT transfected with Wee1 was least Conclusion:Target cells transfected with Wee1 could resist CTL mediated cytotoxity, and anti perforin antibody obviously increased the effect更多还原