【摘要】 目的 :克隆藏猪白细胞介素 4基因cDNA。方法 :从体外ConA刺激 70小时的藏猪外周血淋巴细胞中提取总RNA ,应用RT PCR技术扩增 ,pMD T载体连接 ,常规转化后 ,进行酶切及序列测定进行鉴定。结果 :研究表明克隆得到的IL 4基因cDNA与成华猪的IL 4同源性达到 99% ,与长白杂交猪的同源率为 98%。结论 :从藏猪外周血淋巴细胞中成功地分离到IL 4基因。更多还原

【Abstract】 Objective:To clone interleukin 4 cDNA of Tibet pig.Methods:To amplify cDNA,using RT PCR from the total RNA from the lymphocyte stimulated by 10 μg/ml Con A in vitro,to subclone into pMD T vector,and then to determine it by digesting and sequencing.Results:The cloned Tibet pig interleukin 4 was 98% homologus to the necleotide acids of Chenghua pig a special specie of pig in Sichuan province and 99% homologus to that of hybrid of Landrance with Chanbai pig.Conclusion:IL 4 of Tibet pig was successfully cloned from the lymphocyte after stimulating 70 h with ConA.更多还原