【摘要】 目的:目的:观察狼疮肾炎(LN)外周血单个核细胞(PBMCs)白细胞介素16(IL-16)的分泌及IL-16mRNA的表达。方法:采用酶联免疫吸附法(ELISA)检测PBMCs IL-16分泌的水平;采用逆转录聚合酶链反应(RT-PCR)方法检测PBMCs IL-16mRNA的表达。结果:①LN患者PBMCs自发分泌IL-16,活动组较静止组、正常对照组增高,存在显著性差异(265.6±102.2vs.120.5±84.1;265.6±102.0 vs.50.9±32.4,P<0.05);②LN活动组与静止组PBMCs IL-16mRNA表达增强,较正常对照组存在显著性差异(5.090±1.815 vs.1.030 ±0.426;3.910±1.430 vs.1.030±0.426,P<0.05),但LN活动组与静止组之间IL-16-mRNA的表达差异没有显著性(5.090 ±1.815 vs.3.910±1.430)。结论:LN患者PBMCs存在IL-16mRNA的高表达和自发的IL-16过度分泌。IL-16可能参与介导LN的发病机制。更多还原

【Abstract】 ObjectivetTo investigate the interleukin-16 (IL-16) mRNA expression and IL-16 secretion by peripheral blood mononucle-ar cells (PBMCs) of patients with lupus nephritis (LN) .Methods: 15 patients with active LN, 10 with inactive LN and 8 normal controls were included in the research. TTie supernatant IL-16 levels of freshly isolated PBMCs were measured by ELISA; the IL-16mRNA level were measured by RT-PCR.Results: As compared with the control group, both active and inactive LN patients had significantly increase in IL-16mRNA expression and IL-16 secretion, but didn’t find the difference of IL-16mRNA expression between active and inactive LN groups.Conclusion: LN patients can endogeneous over-express IL-16 gene and abnormally produce IL-16 protein by PBMCs . IL-16 might play a role in the patho-genesis of LN.更多还原