【摘要】 目的 :肝癌相关基因HCAP1蛋白产物的表达、抗体的制备及其亚细胞定位。方法 :原核表达HCAP1蛋白 ;纯化的HCAP1蛋白免疫新西兰大白兔 ,制备多抗血清 ;Westernblot分析其在肝癌细胞株的表达 ;通过荧光免疫细胞化学进行亚细胞定位。结果 :获得了较纯的HCAP1表达蛋白和高效价的、高特异性的抗HCAP1的多抗血清 ,并且发现HCAP1大部分位于胞浆 ,少量分布于核仁。结论 :表达的HCAP1蛋白和抗HCAP1的多抗血清可用于HCAP1基因功能的研究 ;HCAP1功能场所可能位于核仁。更多还原

【Abstract】 Objective:To obtain anti HCAP1 polyclonal antiserum and define subcellular localization of HCC associated protein(HCAP1).Methods:The HCAP1 was expressed in E.coli DE3 cells and purified.The antiserum was prepared by immunizing rabbit with purified HCAP1 protein.The expression of HCAP1 was analyzed by Western blot in the HCC cells lines.The subcellular localization was observed using fluoroimmunocytochemistry.Results:The rabbit antiserum was obtained and HCAP1 was localized in cytoplasm and nucleoli.Conclusion:The expressed HCAP1 and anti HCAP1 antiserum could be used for studying the HCAP1 role in the HCC;The expression pattern of HCAP1 was mainly in cytoplasm and it was also detected in the nucleoli.更多还原