【摘要】 本文研究了普鲁兰酶和β-淀粉酶对大米支链淀粉回生的影响。通过用DSC和α-淀粉酶两种方法测定其回生,结果表明:β-淀粉酶能够通过切短大米支链淀粉外侧枝链而抑制其回生,且随着酶解度的增加回生抑制更加明显。普鲁兰酶的适度处理加快了大米支链淀粉的回生,脱支下来的短直链不能形成结晶,除外侧短枝链外,其它较长链也可参与结晶。两种检测方法所得结果比较一致。更多还原

【Abstract】 The effect of pululanase and β - amylase on the retrogradation of rice amylopectin has been investigated by DSC and α - amylase - iodine methods. the results indicate that β - amylolyse can inhibite the retrogradation by shortening the average external chain length of rice amylopectin and that the β- amylolysis extent is positively correlated to the inhibiting retrogradation. The pullulanase increases the retrogradation. The short amyloses debranched from the amylopectin by the pullulanase can not crystallize, but the longer chain except the short external chain can still crystallize. The results by DSC and α- amylase - iodine method are consilient.更多还原