【摘要】 目的:探讨不同因素对人外周血单核细胞来源的树突状细胞(DC)体外分化成熟的影响。方法:通过流式细胞仪表型分析、混合淋巴细胞反应、抗原摄取能力检测、DC对T细胞的趋化能力及对IL-10的拮抗效应的测定,研究了TNF-α,FL,sCD40L,CD40mAb,gp130,IL-10等不同因素对DC的体外分化成熟及功能的影响。结果;CD40信号和TNF-α都可以有效地使DC上调表达共刺激分子CD80,CD86并进而促进DC激发T细胞的增殖和活化及对T淋巴细胞的趋化作用,但在体外培养体系中,sCD40L能有效地站抗IL-10的抑制效应,而TNF-α则明显不如sCD40L有效;激发型gp130单克隆抗体和人重组FL可以显著地促进DC的体外扩增,但是无明显地促进DC分化成熟和促进DC激发T细胞的功能。结论:CD40和TNF-α都具有促进DC分化、成熟的能力,但CD40的作用明显优于TNF-α。更多还原

【Abstract】 Objective: To study the affecting factors on the differentiation, maturation and function on the human pe-ripheral blood monocyte-derived dendritic cells (DC). Methods: Through DCs’ surface molecules analysis, mixed-lym-phocyte reaction, FITC-Dextran capture, cell counting and chemotaxis activity of T lymphocytes, we compared functions ofDC stimulated with different cytokines including TNF-α, FL, sCD40L, CD40mAb, gp130 and IL-10. Results: BothCD40- and TNF-α signalling are able to promote differentiation and maturation of DC, including upregulation of costimula-tory molecules expression, such as CD80 and CD86, enhancement of DC-mediated MLR, promotion of T cell chemotacticability to DC; CD40 signalling counteracted the inhibitory effects of IL-10 on DC more efficiently than TNF-α signalling;The agonist gp130 mAb and human recombinant FL can remarkably promote the proliferation of DC in vitro, while thereare no distinct effects on DCs’ differentiation, maturation and ability to activate T cells. Conclusions: CD40 signalling ismore powerful than TNF-α, and plays a unique role to abtain more mature and functional DC in vitro.更多还原