【摘要】 目的 :探讨嵌合抗CD2 0抗体Fab′片段的抗肿瘤机制。方法 :应用MTT法检测Fab′片段对Raji细胞生长的抑制作用 ,用AnnexinV FITC和PI测定Fab′片段对Raji细胞凋亡的诱导作用 ,用RT PCR和Westernblot检测Raji细胞中bcl 2表达的变化。结果 :嵌合抗CD2 0Fab′片段对Raji细胞具有明显的抑制作用IC5 0值为 2 4 .2 μg/ml,并呈剂量依赖性 ;嵌合抗CD2 0Fab′片段能诱导Raji细胞的凋亡 ,并降低Raji细胞中bcl 2基因及其蛋白的表达水平。结论 :嵌合抗CD2 0抗体片段Fab′能抑制Raji细胞生长和诱导细胞凋亡 ,其作用机制与bcl 2蛋白表达的下降有关。更多还原

【Abstract】 Objective: To study anti-tumor mechanism of chimeric anti-CD20 antibody fragment Fab’ in vitro . Methods: The effect of anti-CD20 antibody fragment Fab’ on Raji cells growth was observed using MTT; Annexin V-FITC and PI were used to assay Raji cells apoptosis induced by Fab’; bcl-2 gene expression level change in Raji cells induced by Fab’ was assayed using RT-PCR and Western blotting. Results: The result of MTT indicated that the growth of Raji cells was inhibited by chimeric anti-CD20 antibody fragment Fab’ (IC50, 24.2_g/ml); bcl-2 gene expression level in Raji cells was decreased prominently by Fab’. Conclusions: It is relative between growth inhibition of Raji cells and apoptosis induced by chimeric anti-CD20 antibody fragment Fab’, and bcl-2 level decline was involved.更多还原