【摘要】 目的 通过多省市流行病学调查,探讨绝经后妇女血清性激素水平与外周血T淋巴细胞亚群的变化及两者之间的关系。方法 以816例健康绝经后妇女为研究对象,369例未闭经妇女为对照组,用放射免疫法测定血清促卵泡激素（FSH）、黄体生成素（LH）、垂体泌乳素（PRL）、睾酮（T）、游离睾酮（FT）、17-β雌二醇（17β-E₂）及孕酮（P）,用流式细胞仪测定T淋巴细胞亚群。结果 与对照组相比,绝经后血清17β-E₂,P水平明显下降,闭经后17β_E2仍有下降趋势,>70岁P有所回升,E₂/P比值随增龄明显下降。闭经后T明显升高,但FT变化不明显。血清FSH,LH浓度随增龄增加,绝经后各组与对照组相比,差异均有显著性意义,但闭经后各组间差异无显著性意义。PRL值有所下降,但各组间差异无显著性意义。经线性相关分析,17β-E₂,E₂/P与年龄呈负相关（r=-0.472,P<0.001;r=-0.221,P<0.001）。CD₃⁺,CD₄⁺在≤60岁与对照组相比变化不明显,>60岁CD₃⁺有所下降,CD₄⁺则明显下降,CD₈⁺绝经后各组与对照组相比无变化,CD₄⁺/CD₈⁺比值随增龄明显下降。经线性相关分析CD₃⁺,CD₄⁺与年龄呈负相关（r=-0.224,P<0.05;r=-0.298,P<0.001）。经线性相关分析CD₃⁺,CD₄⁺与E₂呈正相关（r=0.356,P<0.001;r=0.454,P<0.001）,而CD₈⁺与E₂无相关。以年龄、体重指数更多还原

【Abstract】 Objective To investigate the correlation between changes in serum sex hormones and peripheral T cell subsets in postmenopausal women. Methods The levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin(PRL) , 17β-estradiol(17β-E2 ) , progesterone (P), testosterone (T) and free testosterone (FT) were measured by radioimmunoassay method, and T cell subsets by flow cytometer. Results Compared with the control group, the 17β-E2 and P levels of post-menopausal groups reduced significantly, and 17β-E2 still had a decrease trend after menopause, but P rose again over 70, correspondingly. The ratio of E2/P reduced significantly with aging. The level of T increased significantly after menopause, but FT remained unchanged. The levels of FSH and LH increased significantly after menopause , but there were no differences between postmenopausal groups . The level of PRL had a decrease trend , but not obvious. 17β-E2 and E2/P were negatively correlated with age respectively ( r = - 0. 472, P < 0. 001; r = -0.221, P<0.001). Compared with the young groups, CD3+ and CD4+ did not change until 60, but reduced significantly after 60. CC8+ had no differences in all groups. The ratio of CD4 + /CD8+ reduced significantly with aging, CD/ and CD4 + presented negative correlations with age( r = - 0. 224, P < 0.05; r = - 0. 298, P<0.001). By bi variate correlation analysis, there were positive correlations between E2 and CD3+ , CD4+ respectively (r = 0.356, P<0.001;r = 0.454, P<0.001),but not was CD8+. Multiple regression analysis showed that CD3+ and CD4+ only had correlations with age and E2 . In order to study the relation between changes in T cell subsets and E2 , the level of E2 was stratified according to the quartile (15.66, 27.05, 57. 80pmol/L), and the data showed that CD3+ ,CD4+ and CD4+/CD8+ reduced significantly with decrease in E2. Conclusions These results indicated that the changes in T cell subsets in the peripheral blood might be correlated with the deficiency of E2 in postmenopausal women.更多还原