【摘要】 目的观察中药复方脑复聪颗粒对H2 O2 损伤的大鼠肾上腺髓质嗜铬瘤分化细胞株 (PC12细胞 )的影响。方法选用SD雄性大鼠 ,分别灌胃脑复聪和蒸馏水后制备血清。培养的PC12细胞加入不同血清孵育后 ,再加入不同浓度的超氧化合物H2 O2造成拟缺血模型 ,采用MTT法观察细胞生存率。结果对受不同剂量H2 O2 损伤的PC12细胞 ,脑复聪含药血清可明显提高其生存率 (P <0 0 5— 0 0 1) ;加入脑复聪血清后的神经细胞可明显抵抗H2 O2 所致的损伤 ,细胞形态基本恢复正常。结论脑复聪颗粒对H2 O2 损伤的PC12细胞有明显保护作用。更多还原

【Abstract】 ObjectiveTo examine effects of traditional Chinese herbs Naofucong grain on PC12 cells injured by H 2O 2 in vitro MethodsRats random divided into 2 groups: Naofucong grain treating group (administered with Naofucong grain) and control group (administered with distilled water) After 3 days administration, serums were obtained and added to cultured PC12 cells which were under different concentrations of H 2O 2 Morphological examinations in PC12 cells were detected by MTT measurement ResultsMTT measurement showed that Naofucong grain administered rats serums significantly increased the survival number of PC12 cells in different concentrations of H 2O 2.Conclusion Naofucong grain may have obvious protective effects on PC12 cells injured by H 2O 2.更多还原