【摘要】 目的 探讨脑淀粉样血管病性出血 (CAAH)与载脂蛋白E(ApoE)基因多态性的关系。方法 应用聚合酶链反应—限制性长度多态技术 (PCR -RFLP)检测了112例脑淀粉样血管病性出血患者 (患者组 )和120例健康志愿者 (对照组 )的ApoE基因和等位基因 ,同时检测他们的血脂、脂蛋白和载脂蛋白等。结果 患者组ApoE基因型ε2/2、ε2/3、ε2/4、ε3/3、ε3/4和ε4/4的频率分别为1.8%、46.4%、8.9 %、19.6%、19.6%和3.6% ,ApoE等位基因ε2、ε3和ε4的频率依次为29.5 %、52.7 %和17.8% ;而对照组分别为1.7%、15.0%、3.3 %、63.3%、16.7%、0,10.8%、79.2%和10.0 % ;两组之间的差别具有显著性意义 (均P=0.0001)。与对照组相比较 ,患者组ApoE基因ε2/3和等位基因ε2的频率明显增高 ,差别具有显著性意义 (均P=0.0001) ;而ε3/3、ε3的频率则明显降低 ,差别具有显著性意义 (均P=0.0001) ;ApoEε基因型2/3和ApoE等位基因ε2的相对危险度及其95%可信区间分别为4.911(2.632～9.163)和3.438(1.700～6.955)。 结论 ApoE等位基因ε2在CAAH患者中出现的频率显著增高 ,很可能是引发CAAH潜在的危险因子。更多还原

【Abstract】 Objective To study on the relationship between the cerebral amyloid angiopathy-related hemorrhage and the polymorphism of apolipoprotein E(Apo E)gene.Methods The polymerase chain reacˉtion-restriction fragment length polymorphism(PCR-RFLP)technique was used to detect frequencies of Apo E genotype and Apo E allele in112patients with cerebral amyloid angiopathy-related hemorrhage(CAAH)(the patient group)and120healthy volunteers(the control group).At the same time,their levels of lipids,lipoprotein and apolipoprotein in serum were determined.Results The frequency of Apo E genotypeε2/2,ε2/3,ε2/4,ε3/3,ε3/4andε4/4in the patient group was1.8%,46.4%,8.9%,19.6%,19.6%and3.6%and the frequency for Apo E alleleε2,ε3,ε4was29.5%,52.7%and17.8%respectively;while those in the control group were1.7%,15.0%,3.3%,63.3%,16.7%,0,10.8%,79.2%and10.0%respectively,the differences were significantly(P=0.0001).Compared with the control group,the frequency ofε2/3andε2was increased(P=0.0001),and those ofε3/3andε3were decreased(P=0.0001).The relative risk(RR)of Apo Eε2/3and Apo Eε2were4.911(95%CI2.632～9.163)and3.438(95%CI1.700～6.955)respectiveˉly.Conclusion The frequency of Apo E alleleε2in the CAAH patients markedly increased,and Apo E alleleε2was likely to be a potential risk factor of CAAH.更多还原