【摘要】 目的 :研究透明质酸钠 (sodium hyaluronate)对大肠癌细胞生长和粘附的影响。方法 :用人类大肠癌细胞SW6 2 0和 Colo2 0 5与透明质酸钠溶液 (2 5～ 2 5 0 0μg/ ml)体外培养 ,然后用 MTT方法测定增殖情况。同时 ,运用流式细胞术 ,对 SW6 2 0和 Colo2 0 5细胞表面的 CD4 4表达进行检测 ,比较不同浓度透明质酸钠溶液对肿瘤粘附力的影响。结果 :MTT结果提示 ,实验组 SW6 2 0细胞生长与对照组无显著差异 ,但实验组 Colo2 0 5细胞生长与对照组有显著差异。另外 ,流式细胞术的结果显示 ,透明质酸钠可使 SW6 2 0和 Colo2 0 5细胞表面的 CD4 4表达下调。结论 :浓度为 2 5～ 2 5 0 0μg/ ml透明质酸钠对不同大肠癌细胞系生长有不同的影响 ,但可降低肿瘤细胞粘附分子的表达 ,从而影响其粘附力。更多还原

【Abstract】 Objective: To investigate the effects of sodium hyaluronate on the growth and adhesion of colorectal cancer cells. Methods: Human colorectal cancer cell lines SW620 and Colo205 were treated with sodium hyaluronate (25~ 2 500 μg/ml),and cancer cell proliferation was measured by MTT assay in vitro.Flow-cytometric analysis was applied to detect expression of CD44 on SW620 and Colo205 cells. Results: In vitro sodium hyaluronate enhanced proliferation of Colo205 cells,but it had no appreciable effect on SW620 growth under the same doses.Meantime,CD44 expression on cancer cells decreased compared with controls. Conclusion: In vitro sodium hyaluronate has different effects on growth of different colorectal cancer cell lines, but can inhibit CD44 expression of colorectal cancer cells and influence their ability of adhesion.更多还原