【摘要】 目的 :建立一种能有效地评价大鼠空间记忆能力的实验模型。方法 :交替以 Y型迷宫的左、右相邻臂作为起始臂和目标臂 ,给予动物足底电刺激作为学习记忆的强化因子 ,并通过测定动物腹腔内注射东莨菪碱 (0 .5mg/ kg)或双侧海马内微量注射 MK- 80 1(0 .2 μg/ site)诱发的大鼠空间记忆障碍来验证该模型的可靠性。结果 :正常组、手术对照组或生理盐水组大鼠均能迅速学会左右交替的记忆模式 ,而东莨菪碱或 MK- 80 1会导致大鼠在 Y迷宫中的空间记忆能力 (包括工作记忆和参照记忆 )的障碍 (P<0 .0 5 ) ,而且给药组和对照组使用的电刺激强度没有明显的差异 (P>0 .0 5 )。结论 :交替电刺激 Y迷宫是一种能比较客观且有效地评价大鼠空间记忆能力的模型。更多还原

【Abstract】 Objective: To establish an effective model for evaluating the spatial memory of rats. Methods: The adjacent left or right arm in Y maze was defined as an initial arm or an objective one alternatively, and electro stimulus to vola was used as a reinforcement. Meanwhile, the reliability of the model was validated by measuring the memory deficits induced by intraperitoneal (ip) injection of scopolamine (0.5 mg/kg) or bilaterally intrahippocampal (ih) microinjection of MK 801(0.2 μg/site). Results: The normal, sham operated or saline treated rats learned the left right alternative memory model rapidly while injection of either scopolamine or MK 801 caused spatial memory deficits in Y maze, including deficits in working and reference memory. Moreover, there was no appreciable difference in electricity intensity between the treated group and the control group. Conclusion: The alternative Y maze with electro stimulus is a comparatively objective model which can evaluate the spatial memory in rats effectively.[更多还原