【摘要】 目的　用 mt CO 技术鉴定云南省大理及贵州省都匀、从江等地所发现的是牛带绦虫或牛带绦虫亚洲亚种。　方法　采集成虫样本 ,抽提 DNA,PCR扩增线粒体细胞色素 C氧化酶 (mt CO )部分基因片段并测序 ,经PHYL IP软件包处理 ,计算遗传距离并构建系统发育树状图。　结果　云南省兰坪、大理和贵州省都匀的标本 mt CO 基因片段序列与已知牛带绦虫亚洲亚种的序列相同。贵州从江的标本 mt CO 基因片段序列与已知牛带绦虫的序列相同。两组基因片段的同源性达 97.4 4 % ,氨基酸序列同源性达 99.16 %。系统发育树状图显示 ,牛带绦虫亚洲亚种和牛带绦虫最为接近 ,远离猪带绦虫及其他绦虫。　结论　云南省兰坪、大理及贵州省都匀存在牛带绦虫亚洲亚种 ,贵州省从江发现的虫种为牛带绦虫。更多还原

【Abstract】 Objective To identify the types of Taenia saginata isolated from Dali of Yunnan Province, and from Duyun and Congjiang of Guizhou Province.\ Methods\ Genomic DNA was isolated, and the mitochondrial cytochrome C oxidase subunitⅠ(mtCOⅠ) genes were amplified by polymerase chain reaction(PCR) and analyzed by PHYLIP software package.\ Results \ The mtCOⅠgene sequences of Lanping sample were identical to that of T\^saginata asiatica known in Taiwan, and the samples obtained from Dali and Duyun showed the same mtCOⅠgene sequences, while the sample from Congjiang had the same mtCOⅠgene sequences as T\^saginata . The homology between these two groups of gene sequences was 97\^44%, while the homology of amino acid sequences reached to 99\^16%. The constructed phylogenetic tree revealed that the relationship between T\^saginata asiatica and T\^saginata is closer, both are distant relative to T\^solium and other species of cestodes.\ Conclusion \ The Taenia prevalent in Lanping, Dali and Duyun is identified as T\^saginata asiatica , while that isolated in Congjiang is the typical T\^saginata.更多还原