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栝楼性别分化特异大分子标记物研究

Studies on the specific macromolecular markers of sex differentiation in Thrichosanthes kirilowii Maxim

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【作者】 于凤池孙耘子袁高峰汪俏梅

【Author】 YU Fengchi, SUN Yunzi, YUAN Gaofeng, WANG Qiaomei*(Institute of Vegetable, Zhejiang University, Hangzhou 310029,China) 

【机构】 浙江大学蔬菜研究所浙江大学蔬菜研究所 浙江杭州310029浙江杭州310029浙江杭州310029

【摘要】 对栝楼雌、雄株不同器官进行过氧化物酶同工酶活性和电泳分析。结果表明,雌株的过氧化物酶同工酶活性高于雄株,且雌株叶片中有一条特异性的过氧化物酶同工酶谱带;对雌、雄株不同发育时期的花器官进行可溶性蛋白的SDS PAGE分析表明,雄株幼花芽中存在一种23.4kD的特异蛋白质,雄花中一种32.3kD的蛋白质在受精后消失。

【Abstract】 The peroxidase isoenzyme activity and eletrophoresis analysis of various organs of male and female Thrichosanthes kirilowii Maxim plants were studied. The results indicated that the peroxidase isoenzyme activity in female plants was higher than that in male plants,and there was a specific peroxidase isoenzyme band in the leaf of female plants. SDSPAGE analysis of soluble proteins in male and female flowers at various developmental stages shown that there was a specific 23.4 kD protein in male flower bud, and a 32.3 kD protein in male flower disappeared after fertilization.

【基金】 国家自然科学基金(30000015);浙江省教育厅资助项目。
  • 【文献出处】 浙江农业学报 ,Acta Agriculturae Zhejiangensis , 编辑部邮箱 ,2003年06期
  • 【分类号】Q946
  • 【被引频次】39
  • 【下载频次】189
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